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Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
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Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

6,714 Views

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07:37 min

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February 21, 2020

DOI:

10.3791/60868-v

DOI:
10.3791/60868-v

•

07:37 min
February 21, 2020

•

2 Views
, , ,
1College of Animal Science & Technology,Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Summary

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Several different methods have been established for multiplex immunostaining using primary antibodies from the same host species. Here, we describe the use of microwave-mediated antibody stripping and of fluorophore-tyramide to block antibody cross-reactivity during multiplex immunostaining on formalin-fixed paraffin-embedded mouse adrenal sections.

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Cite this Article

Lyu, Q., Zheng, H. S., Laprocina, K., Huang, C. J. Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species. J. Vis. Exp. (156), e60868, doi:10.3791/60868 (2020).

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