Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals &#8722; Using Unconjugated Primary Antibodies from the Same Host Species

Qiongxia Lyu; Huifei  Sophia Zheng; Karly Laprocina; Chen-Che Jeff Huang

doi:10.3791/60868

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Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

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発生生物学

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JoVE Journal 発生生物学

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

6,714 Views

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07:37 min

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February 21, 2020

DOI:

10.3791/60868-v

DOI:

10.3791/60868-v

•

07:37 min

February 21, 2020

•

2 Views

Qiongxia Lyu^1,2, Huifei Sophia Zheng², Karly Laprocina², Chen-Che Jeff Huang²

¹College of Animal Science & Technology,Henan University of Science and Technology, ²Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

概要

Several different methods have been established for multiplex immunostaining using primary antibodies from the same host species. Here, we describe the use of microwave-mediated antibody stripping and of fluorophore-tyramide to block antibody cross-reactivity during multiplex immunostaining on formalin-fixed paraffin-embedded mouse adrenal sections.

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Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

概要

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