Journal
/
화학
/
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
JoVE 신문
화학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

8,891 Views

•

15:03 min

•

June 16, 2020

DOI:

10.3791/60842-v

DOI:
10.3791/60842-v

•

15:03 min
June 16, 2020

•

8 Views
, , , , , ,
1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias,Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación,Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture,Universidade de Santiago de Compostela

Summary

Automatically generated

This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.

Explore More Videos

Magnetic NanoparticlesSilica CoatingAmine FunctionalizationSiderophore FeroxamineBacteria DetectionIron UptakeNanoparticle SynthesisCore-shell StructureSurface ModificationAnalytical ScienceNanomedicine

Read Article

Cite this Article

Martínez-Matamoros, D., Castro-García, S., Ojeda Romano, G., Balado, M., Rodríguez, J., Lemos, M. L., Jiménez, C. Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection. J. Vis. Exp. (160), e60842, doi:10.3791/60842 (2020).

Download .ris file

Copy

Share Video

.

Copy