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Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
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化学
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JoVE Journal 化学
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

8,891 Views

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15:03 min

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June 16, 2020

DOI:

10.3791/60842-v

DOI:
10.3791/60842-v

•

15:03 min
June 16, 2020

•

8 Views
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1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias,Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación,Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture,Universidade de Santiago de Compostela

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This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.

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Martínez-Matamoros, D., Castro-García, S., Ojeda Romano, G., Balado, M., Rodríguez, J., Lemos, M. L., Jiménez, C. Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection. J. Vis. Exp. (160), e60842, doi:10.3791/60842 (2020).

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