Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.
Li, J., Ünal, C. M., Namikawa, K., Steinert, M., Köster, R. W. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (156), e60793, doi:10.3791/60793 (2020).