Journal
/
생화학
/
Ubiquitin Chain Analysis by Parallel Reaction Monitoring
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Ubiquitin Chain Analysis by Parallel Reaction Monitoring

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

DOI:
10.3791/60702-v

•

08:33 min

•

June 17, 2020

•

, ,
1Quantitative Biology Unit,Luxembourg Institute of Health

Chapters

  • 00:05Introduction
  • 00:51Sample Preparation
  • 04:04Software Analysis
  • 05:49Results: Derivation of the Ubiquitin Topology-characteristic Peptide
  • 07:44Conclusion

Summary

자동 번역

자동 번역

This method describes the assessment of global changes in ubiquitin chain topology. The assessment is performed by the application of a mass spectrometry-based targeted proteomics approach.

Tags

Ubiquitin Chain AnalysisParallel Reaction MonitoringPRMUbiquitin Chain TopologyBiological StatesModified PeptidesMass SpectrometryAmmonium BicarbonateN-ethylmaleimideCell LysisTCEPChloroacetaldehyde

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Read Article