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Ubiquitin Chain Analysis by Parallel Reaction Monitoring
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JoVE Journal 生化学
Ubiquitin Chain Analysis by Parallel Reaction Monitoring

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

DOI:
10.3791/60702-v

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08:33 min

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June 17, 2020

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, ,
1Quantitative Biology Unit,Luxembourg Institute of Health

章

  • 00:05Introduction
  • 00:51Sample Preparation
  • 04:04Software Analysis
  • 05:49Results: Derivation of the Ubiquitin Topology-characteristic Peptide
  • 07:44Conclusion

概要

自動翻訳

自動翻訳

This method describes the assessment of global changes in ubiquitin chain topology. The assessment is performed by the application of a mass spectrometry-based targeted proteomics approach.

タグ

Ubiquitin Chain AnalysisParallel Reaction MonitoringPRMUbiquitin Chain TopologyBiological StatesModified PeptidesMass SpectrometryAmmonium BicarbonateN-ethylmaleimideCell LysisTCEPChloroacetaldehyde

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