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通过生命内显微镜在斑马鱼胚胎的分孔细菌感染过程中巨噬细胞细胞死亡的可视化
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy
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通过生命内显微镜在斑马鱼胚胎的分孔细菌感染过程中巨噬细胞细胞死亡的可视化

DOI:
10.3791/60698-v

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06:49 min

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January 09, 2019

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, , , , , ,
1Shanghai Public Health Clinical Center,Fudan University, 2School of Life Sciences,Bengbu Medical College

Chapters

  • 00:04Title
  • 00:55M. marinum Single Cell Inoculum Preparation
  • 02:01Infection via Bacterial Microinjection
  • 04:16Live Imaging of the Infection
  • 05:13Results: Morphological Changes in M. marinum Infection Induced Macrophage Lytic Cell Death and UV Induced Macrophage Apoptosis
  • 06:19Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种技术，用于可视化在胚胎斑马鱼的巨噬行为和死亡在分枝杆菌感染期间。包括制备细菌、胚胎感染和生命内显微镜的步骤。在涉及感染或无菌炎症的类似情况下，此技术可应用于观察细胞行为和死亡。

Tags

MacrophageLytic Cell DeathMycobacterial InfectionZebrafish EmbryosIntravital MicroscopyCell Death VisualizationLive ImagingMycobacterium MarinumAgarose MountingIntramuscular Infection

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