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Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy
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Chapitres
- 00:04Titre
- 00:55M. marinum Single Cell Inoculum Preparation
- 02:01Infection via Bacterial Microinjection
- 04:16Live Imaging of the Infection
- 05:13Results: Morphological Changes in M. marinum Infection Induced Macrophage Lytic Cell Death and UV Induced Macrophage Apoptosis
- 06:19Conclusion
This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation.
