Journal
/
면역학 및 감염병학
/
Live Cell Imaging en Cryo-Electron Tomography toepassen op Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Please note that all translations are automatically generated. Click here for the English version.

Live Cell Imaging en Cryo-Electron Tomography toepassen op Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

6,663 Views

•

09:12 min

•

March 10, 2020

DOI:

10.3791/60693-v

DOI:
10.3791/60693-v

•

09:12 min
March 10, 2020

•

3 Views
, , , ,
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine,Yale University School of Medicine, 2Microbial Sciences Institute,Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School,The University of Texas Health Science Center at Houston

Summary

Automatically generated

Beeldvorming van bacteriële cellen is een opkomende systeembiologiebenadering gericht op het definiëren van statische en dynamische processen die de functie van grote macromoleculaire machines dicteren. Hier wordt integratie van kwantitatieve live celbeeldvorming en cryo-elektronentomografie gebruikt om legionella pneumophila type IV secretiesysteemarchitectuur en -functies te bestuderen.

Explore More Videos

Live Cell ImagingCryo-electron TomographyLegionella PneumophilaDot/Icm Secretion SystemBacterial Secretion ComplexesHost-pathogen InteractionsStructural-functional RelationshipsAgarose PadsFluorescence MicroscopyPolarity Quantification

Read Article

Cite this Article

Chetrit, D., Park, D., Hu, B., Liu, J., Roy, C. R. Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System. J. Vis. Exp. (157), e60693, doi:10.3791/60693 (2020).

Download .ris file

Copy

Share Video

.

Copy