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Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
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Immunology and Infection
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JoVE Journal Immunology and Infection
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

English

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6,663 Views

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09:12 min

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March 10, 2020

DOI:

10.3791/60693-v

DOI:
10.3791/60693-v

•

09:12 min
March 10, 2020

•

3 Views
, , , ,
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine,Yale University School of Medicine, 2Microbial Sciences Institute,Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School,The University of Texas Health Science Center at Houston

Summary

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Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.

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Cite this Article

Chetrit, D., Park, D., Hu, B., Liu, J., Roy, C. R. Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System. J. Vis. Exp. (157), e60693, doi:10.3791/60693 (2020).

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