Rivista
/
Immunologia e infezioni
/
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

DOI:
10.3791/60693-v

•

09:12 min

•

March 10, 2020

•

, , , ,
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine,Yale University School of Medicine, 2Microbial Sciences Institute,Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School,The University of Texas Health Science Center at Houston

Capitoli

  • 00:04Introduction
  • 00:55Live Cell Imaging of L. pneumophila with Fluorescently Tagged Dot/Icm Components
  • 02:47Quantification of Polar Localization and Dynamics of Dot/Icm Components
  • 05:36Detection of sfGFP Mass Density with Cryo-ET
  • 06:23Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria
  • 08:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.

Tags

Keywords: Live Cell ImagingCryo-electron TomographyLegionella PneumophilaDot/Icm Secretion SystemBacterial Secretion ComplexesHost-pathogen InteractionsStructural-functional RelationshipsAgarose PadsFluorescence MicroscopyPolarity Quantification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60693?article_type=v

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
check_url/it/60693?article_type=v

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
check_url/it/60693?article_type=v

Real-time Live Imaging of T-cell Signaling Complex Formation
check_url/it/60693?article_type=v

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
check_url/it/60693?article_type=v

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
check_url/it/60693?article_type=v

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
check_url/it/60693?article_type=v

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
check_url/it/60693?article_type=v

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
check_url/it/60693?article_type=v

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
check_url/it/60693?article_type=v

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

Read Article