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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
JoVE 신문
생화학
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JoVE 신문 생화학
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

DOI:
10.3791/60602-v

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07:18 min

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May 21, 2020

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, , ,
1Department of Chemistry and Biotechnology,Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster,International Rice Research Institute

Chapters

  • 00:04Introduction
  • 00:40Microarray Hybridization
  • 03:09Microarray Scanning
  • 05:05Results: Representative Transcriptome Data Analyses
  • 06:39Conclusion

Summary

자동 번역

자동 번역

A method for transcriptome profiling of cereals is presented. The microarray-based gene expression profiling starts with the isolation of high-quality total RNA from cereal grains and continues with the generation of cDNA. After cRNA labelling and microarray hybridization, recommendations are given for signal detection and quality control.

Tags

Microarray HybridizationGene Expression ProfilingCereal SeedsTranscriptome DataHigh-throughput SequencingBlocking AgentFragmentation MixHybridization BufferMicroarray SlideHybridization AssemblyHybridization Oven

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