/
/
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
This content is Free Access.
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
7,109 Views
•
07:18 min
•
May 21, 2020
DOI:
1Department of Chemistry and Biotechnology,Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster,International Rice Research Institute
Summary
Automatically generated
A method for transcriptome profiling of cereals is presented. The microarray-based gene expression profiling starts with the isolation of high-quality total RNA from cereal grains and continues with the generation of cDNA. After cRNA labelling and microarray hybridization, recommendations are given for signal detection and quality control.
Read Article
Cite this Article
Butardo Jr., V. M., Seiler, C., Sreenivasulu, N., Kuhlmann, M. Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling. J. Vis. Exp. (159), e60602, doi:10.3791/60602 (2020).
Copy