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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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JoVE 신문 면역학 및 감염병학
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

DOI:

08:02 min

January 22, 2020

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Chapters

  • 00:05Title
  • 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
  • 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
  • 05:06BMDC Lenti-CYP-ALDH Transduction
  • 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
  • 07:38Conclusion

Summary

자동 번역

Ici nous présentons un protocole pour l'augmentation in vivo de l'induction réglementaire de cellule T d'intestin-homing. Dans ce protocole, les cellules dendritiques sont conçues pour produire localement des concentrations élevées de la vitamine Active D (1,25-dihydroxyvitamin D ou 1,25[OH]2D) et de la vitamine A active (acide rétinoïque ou RA) de novo.

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