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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Chapters
- 00:05Title
- 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
- 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
- 05:06BMDC Lenti-CYP-ALDH Transduction
- 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
- 07:38Conclusion
Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.
