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Immunology and Infection

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JoVE Journal Immunology and Infection
NF-κB-afhankelijke Luciferase activering en kwantificering van genexpressie in met salmonella geïnfecteerde weefselkweek cellen
 
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NF-κB-afhankelijke Luciferase activering en kwantificering van genexpressie in met salmonella geïnfecteerde weefselkweek cellen

Article DOI: 10.3791/60567-v • 10:57 min • January 12th, 2020
January 12th, 2020 •
1, 1
1Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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Hier presenteren we een protocol om snel en gemakkelijk te meten nucleaire factor Kappa-Light-Chain-Enhancer van geactiveerde B-cellen (NF-κB) activering in cellijnen die NF-κB:: Luciferase reporter constructies, via metingen van luminescentie in de cel lysaat. Bovendien wordt genexpressie bepaald via RT-qPCR geïsoleerd uit cellen die besmet zijn met salmonella typhimurium.

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Immunologie en infectie uitgave 155 NF-κB Luciferase luminescentie salmonella weefselcultuur immuunrespons GENEXPRESSIE Rt-qPCR
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