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Journal / Immunology and Infection / 在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量…
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Immunology and Infection

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JoVE Journal Immunology and Infection
在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量
 
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在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量

Article DOI: 10.3791/60567-v • 10:57 min • January 12th, 2020
January 12th, 2020 •
1, 1
1Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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在这里，我们提出了一个协议，通过测量细胞裂解中的发光，快速、轻松地测量活性B细胞（NF-B）激活细胞系中激活的核因子kappa-光链增强剂：：快星酶报告器结构。此外，基因表达通过从感染沙门氏菌的细胞中分离的RT-qPCR确定。

Tags

免疫学和感染，第155期，NF-βB，荧光素酶，发光，沙门氏菌，组织培养，免疫反应，基因表达，RT-qPCR
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