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Imaging the Human Immunological Synapse
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December 26, 2019
DOI:
1Instituto de Investigaciones Biomédicas Alberto Sols,CSIC-Universidad Autónoma de Madrid, 2Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina,Universidad Autónoma de Madrid, 3Unidad de Audiovisuales, Facultad de Medicina,Universidad Autónoma de Madrid
Summary
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This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).
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Bello-Gamboa, A., Izquierdo, J. M., Velasco, M., Moreno, S., Garrido, A., Meyers, L., Palomino, J. C., Calvo, V., Izquierdo, M. Imaging the Human Immunological Synapse. J. Vis. Exp. (154), e60312, doi:10.3791/60312 (2019).
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