Imaging the Human Immunological Synapse

Ana Bello-Gamboa; Juan  Manuel Izquierdo; Marta Velasco; Solange Moreno; Alejandro Garrido; Laura Meyers; Juan  Carlos Palomino; V&#237;ctor Calvo; Manuel Izquierdo

doi:10.3791/60312

Journal

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Immunology and Infection

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Imaging the Human Immunological Synapse

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Imaging the Human Immunological Synapse

13,362 Views

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09:37 min

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December 26, 2019

DOI:

10.3791/60312-v

DOI:

10.3791/60312-v

•

09:37 min

December 26, 2019

•

54 Views

Ana Bello-Gamboa*^1,2, Juan Manuel Izquierdo^1,2, Marta Velasco^1,2, Solange Moreno^1,2, Alejandro Garrido^1,2, Laura Meyers^1,2, Juan Carlos Palomino³, Víctor Calvo^1,2, Manuel Izquierdo*^1,2

¹Instituto de Investigaciones Biomédicas Alberto Sols,CSIC-Universidad Autónoma de Madrid, ²Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina,Universidad Autónoma de Madrid, ³Unidad de Audiovisuales, Facultad de Medicina,Universidad Autónoma de Madrid

Summary

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).

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Immunological Synapse Cell-to-cell Conjugation Antigen-presenting Cell T-helper Lymphocyte Time-lapse Acquisition Wide-field Fluorescence Microscopy Image Processing Cell Fixation Immunofluorescence Staining Laser Scanning Fluorescent Microscopy Fibronectin Raji Cells Cell Tracker Blue Cell Suspension

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Imaging the Human Immunological Synapse

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