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Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-
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Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons Time-
Chapters
- 00:04Title
- 00:54Larval Brain Explant Dissection and Mounting
- 02:04Live Time-Lapse Imaging
- 03:02Drift Correction and Deconvolution
- 03:54Image Annotation
- 05:06Dynamic Dendritic Branch Calculation
- 06:43Results: Representative Dynamic Dendritic Branch Terminal Tracking
- 07:34Conclusion
Ici, nous décrivons la méthode que nous avons employée pour imager la filodose dendritique fortement motivoir dans une préparation en direct du cerveau larvaire de Drosophila, et le protocole que nous avons développé pour quantifier des ensembles de données d'imagerie 3D de time-lapse pour des évaluations quantitatives du dendrite dynamique dans le développement des neurones.
