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Neuroscience

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JoVE Journal Neuroscience
Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
 

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Article DOI: 10.3791/60287-v • 08:23 min • September 25th, 2019
September 25th, 2019 •
1, 1, 1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Here, we describe the method we employed to image highly motile dendritic filopodia in a live preparation of the Drosophila larval brain, and the protocol we developed to quantify time-lapse 3D imaging datasets for quantitative assessments of dendrite dynamics in developing neurons.

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Time-lapse Imaging Quantification Dendritic Branch Dynamics Developing Drosophila Neurons 3D Image Annotation Software Dynamic Behaviors Dendritic Filopodia Development And Activity Regulation Sparsely Labeled Neurons In Vitro Settings In Vivo Settings Imaging Parameters Temporal Resolution Spatial Resolution Larval Drosophila Brains Dissecting Microscope Dissection Forceps Brain Lobes Ventral Nerve Cord Attached Muscles Sample Movement Square Chamber External Saline Solution
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