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Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
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JoVE Journal Nörobilim
Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

6,073 Views

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08:23 min

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September 25, 2019

DOI:

10.3791/60287-v

DOI:
10.3791/60287-v

•

08:23 min
September 25, 2019

•

4 Views
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1National Institute of Neurological Disorders and Stroke,National Institutes of Health

Özet

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Here, we describe the method we employed to image highly motile dendritic filopodia in a live preparation of the Drosophila larval brain, and the protocol we developed to quantify time-lapse 3D imaging datasets for quantitative assessments of dendrite dynamics in developing neurons.

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Cite this Article

Sheng, C., Javed, U., Rosenthal, J., Yin, J., Qin, B., Yuan, Q. Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons. J. Vis. Exp. (151), e60287, doi:10.3791/60287 (2019).

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