/

/

単離マウス心室筋細胞の光学イメージング

JoVE 신문
생체공학

This content is Free Access.

JoVE 신문 생체공학

Optical Imaging of Isolated Murine Ventricular Myocytes

Please note that all translations are automatically generated. Click here for the English version.

単離マウス心室筋細胞の光学イメージング

5,951 Views

•

11:32 min

•

January 17, 2020

DOI:

10.3791/60196-v

DOI:

10.3791/60196-v

•

11:32 min

January 17, 2020

•

2 Views

Shuxin Han^1,2, Matt Klos³, Sherry Morgenstern³, Ramiz Ahmad³, Isabella Pua³, Shreyas Suresh¹, Kayla Hicks³, Eric Devaney³

¹Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, ²Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, ³Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center

Summary

マウス筋細胞の単離の方法論と、同時デジタル細胞幾何学的測定と蛍光光光測定を用いたサルコメア短縮トレースと同時に電圧またはカルシウムの痕跡を得る方法を提示する。

Explore More Videos

Optical Imaging Murine Ventricular Myocytes Heart Failure Cardiac Metabolism Excitation-contraction Coupling Cardiac Myocyte Isolation Fluorescent Probe Cardiac Toxicity Heart Failure Therapeutics Transgenic Mice Laminin Langendorf Perfusion KHB-HB Solution Digestion Buffer

Read Article