This content is Free Access.
Optical Imaging of Isolated Murine Ventricular Myocytes
5,954 Views
•
11:32 min
•
January 17, 2020
DOI:
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center
概要
Automatically generated
We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.
Read Article
Cite this Article
Han, S., Klos, M., Morgenstern, S., Ahmad, R., Pua, I., Suresh, S., Hicks, K., Devaney, E. Optical Imaging of Isolated Murine Ventricular Myocytes. J. Vis. Exp. (155), e60196, doi:10.3791/60196 (2020).
Copy