We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.
Han, S., Klos, M., Morgenstern, S., Ahmad, R., Pua, I., Suresh, S., Hicks, K., Devaney, E. Optical Imaging of Isolated Murine Ventricular Myocytes. J. Vis. Exp. (155), e60196, doi:10.3791/60196 (2020).