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Optical Imaging of Isolated Murine Ventricular Myocytes
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January 17, 2020
DOI:
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center
Summary
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We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.
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Han, S., Klos, M., Morgenstern, S., Ahmad, R., Pua, I., Suresh, S., Hicks, K., Devaney, E. Optical Imaging of Isolated Murine Ventricular Myocytes. J. Vis. Exp. (155), e60196, doi:10.3791/60196 (2020).
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