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分离的月毛心室肌细胞的光学成像

JoVE 신문
생체공학

This content is Free Access.

JoVE 신문 생체공학

Optical Imaging of Isolated Murine Ventricular Myocytes

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分离的月毛心室肌细胞的光学成像

5,951 Views

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11:32 min

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January 17, 2020

DOI:

10.3791/60196-v

DOI:

10.3791/60196-v

•

11:32 min

January 17, 2020

•

2 Views

Shuxin Han^1,2, Matt Klos³, Sherry Morgenstern³, Ramiz Ahmad³, Isabella Pua³, Shreyas Suresh¹, Kayla Hicks³, Eric Devaney³

¹Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, ²Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, ³Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center

Summary

我们介绍了分离鼠肌细胞的方法，以及如何使用荧光光度测量与同时进行数字细胞几何测量，同时获得电压或钙痕迹。

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