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正常小鼠和亨廷顿小鼠急性脑切片中谷氨酸释放和服用的单突触指标
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Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
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正常小鼠和亨廷顿小鼠急性脑切片中谷氨酸释放和服用的单突触指标

DOI:
10.3791/60113-v

•

08:27 min

•

March 11, 2020

•

,
1Synaptic Dysfunction Group, Neuroscience Research Center,Charité – University Medicine

Chapters

  • 00:04Introduction
  • 00:28Autofluorescence Correction
  • 01:48Bouton Search
  • 03:19Glutamate Release and Clearance Visualization
  • 04:49Off-Line Glutamate Transient Construction and Rapid Glutamate Release and Clearance Assessment
  • 06:01Results: Representative Identification of Dysfunctional Synapses in Q175 Mice with a Hypokinetic Phenotype
  • 07:51Conclusion

Summary

자동 번역

자동 번역

我们提出了一个协议，以评估成年小鼠急性切片中单皮质谷胱甘肽突触的谷氨酸释放和间隙之间的平衡。该协议使用荧光传感器iGluu进行谷氨酸检测，sCMOS摄像机用于信号采集和用于聚焦激光照明的设备。

Tags

Single SynapseGlutamate ReleaseGlutamate UptakeAcute Brain SlicesHuntington MiceOne-photon MicroscopeAutofluorescence CorrectionDorsal StriatumStimulation PipetteAction Potential Dependent ReleaseIon Channel BlockersMembrane ReceptorsTetrototoxin

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