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Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
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神经科学
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JoVE 杂志 神经科学
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

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08:27 min

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March 11, 2020

DOI:

10.3791/60113-v

DOI:
10.3791/60113-v

•

08:27 min
March 11, 2020

•

4 Views
,
1Synaptic Dysfunction Group, Neuroscience Research Center,Charité – University Medicine

Summary

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We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.

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Dvorzhak, A., Grantyn, R. Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice. J. Vis. Exp. (157), e60113, doi:10.3791/60113 (2020).

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