/
/
Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
6,630 Views
•
09:39 min
•
September 17, 2019
DOI:
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences,Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences,Tsinghua University
Summary
Automatically generated
Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) is novel for the elimination of harmful antibodies. Here, we describe a detailed protocol for the synthesis of DCAF1 molecule, which can selectively block 4G2 antibody to eliminate antibody dependent enhancement effect during Dengue virus infection.
Read Article
Cite this Article
Bai, X., Zhang, L., Hu, J., Zhao, X., Pan, J., Deng, H., Feng, S. Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF). J. Vis. Exp. (151), e60063, doi:10.3791/60063 (2019).
Copy