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Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
Bu İçerik görüntülemek için JoVE aboneliği gereklidir.. Oturum açın veya ücretsiz deneme sürümünü başlatın.
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences,Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences,Tsinghua University
Bölümler
- 00:05Başlık
- 00:57Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
- 02:31Cleavage of the SUMO Tag
- 04:09Assembling of DCAF1 by Native Chemical Ligation
- 07:06Mass Spectrometry and ELISA Assay
- 08:19Results: Native Chemical Ligation and ELISA Assay
- 09:03Conclusion
Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) is novel for the elimination of harmful antibodies. Here, we describe a detailed protocol for the synthesis of DCAF1 molecule, which can selectively block 4G2 antibody to eliminate antibody dependent enhancement effect during Dengue virus infection.
