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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

6,640 Views

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10:25 min

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September 20, 2019

DOI:

10.3791/60060-v

DOI:
10.3791/60060-v

•

10:25 min
September 20, 2019

•

1 Views
,
1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Summary

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In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

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Lymphocyte MigrationTransmigrationImmune ResponseChemokinesILC2Ex VivoCell IsolationCell CountingCell Suspension

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Cite this Article

Warren, K. J., Wyatt, T. A. Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System. J. Vis. Exp. (151), e60060, doi:10.3791/60060 (2019).

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