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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

DOI:
10.3791/60060-v

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10:25 min

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September 20, 2019

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,
1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

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In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

Tags

Lymphocyte MigrationTransmigrationImmune ResponseChemokinesILC2Ex VivoCell IsolationCell CountingCell Suspension

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