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Meten van interacties tussen fluorescentie sondes en lignine in plantendelen door sFLIM op basis van inheemse Autofluorescentie
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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
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Meten van interacties tussen fluorescentie sondes en lignine in plantendelen door sFLIM op basis van inheemse Autofluorescentie

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00:07 min

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January 02, 2020

DOI:

10.3791/59925-v

DOI:
10.3791/59925-v

•

00:07 min
January 02, 2020

•

2 Views
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1PICT Platform,Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

Summary

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Dit protocol beschrijft een originele Setup met een combinatie van spectrale en fluorescentie levensduur metingen om Förster Resonance Energy Transfer (FRET) te evalueren tussen de op Rhodamine gebaseerde fluorescentie sondes en lignine Polymer rechtstreeks in dikke plantendelen.

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Terryn, C., Habrant, A., Paës, G., Spriet, C. Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence. J. Vis. Exp. (155), e59925, doi:10.3791/59925 (2020).

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