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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
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生化学
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JoVE Journal 生化学
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

DOI:
10.3791/59925-v

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00:07 min

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January 02, 2020

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, , ,
1PICT Platform,Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

章

  • 00:04標題
  • 00:54Plant Sample Preparation
  • 02:21Spectrally-resolved Fluorescence Lifetime and Spectral Measurement (sFLIM) System Calibration
  • 03:09sFLIM Measurements
  • 04:13sFLIM Analysis
  • 05:26Results: Representative sFLIM Measurements
  • 06:29Conclusion

概要

自動翻訳

自動翻訳

This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

タグ

Fluorescent ProbesLigninPlant SectionsSFLIMNative AutofluorescenceFRET AnalysisLignocellulosic BiomassEnzyme ActivityPolysaccharidesSample SectioningMultidimensional MicroscopyPEG EmbeddingMicrotomeFluorescent StainingSpectral WindowSFLIM DetectorTitanium-doped Sapphire Laser

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