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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

DOI:
10.3791/59925-v

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00:07 min

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January 02, 2020

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, , ,
1PICT Platform,Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

Kapitel

  • 00:04Titel
  • 00:54Plant Sample Preparation
  • 02:21Spectrally-resolved Fluorescence Lifetime and Spectral Measurement (sFLIM) System Calibration
  • 03:09sFLIM Measurements
  • 04:13sFLIM Analysis
  • 05:26Results: Representative sFLIM Measurements
  • 06:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

Tags

Fluorescent ProbesLigninPlant SectionsSFLIMNative AutofluorescenceFRET AnalysisLignocellulosic BiomassEnzyme ActivityPolysaccharidesSample SectioningMultidimensional MicroscopyPEG EmbeddingMicrotomeFluorescent StainingSpectral WindowSFLIM DetectorTitanium-doped Sapphire Laser

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