Journal
/
생체공학
/
用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
Please note that all translations are automatically generated. Click here for the English version.

用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络

11,146 Views

•

08:53 min

•

July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

Automatically generated

本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。

Explore More Videos

Human IPSC-derived CardiomyocytesMultiwell Micro-electrode ArraysAction Potential RecordingsCell PlatingElectrophysiologyMATLAB WorkflowSignal-to-noise RatioFibronectin Coating

Read Article

Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

Download .ris file

Copy

Share Video

.

Copy