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Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

11,200 Views

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08:53 min

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July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

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This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

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Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

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