Journal
/
생체공학
/
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

11,200 Views

•

08:53 min

•

July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

Automatically generated

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

Explore More Videos

Human IPSC-derived CardiomyocytesMultiwell Micro-electrode ArraysAction Potential RecordingsCell PlatingElectrophysiologyMATLAB WorkflowSignal-to-noise RatioFibronectin Coating

Read Article

Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

Download .ris file

Copy

Share Video

.

Copy