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Purification of High Yield Extracellular Vesicle Preparations Away from Virus
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Chapters
- 00:04Title
- 01:00Filtration and Precipitation of Extracellular Vesicles
- 02:25Construction of a Density Gradient and Enrichment of EV Fractions
- 04:06Preparation of Nanoparticle Pellet for Downstream Assay
- 05:05Results: Increased EV Yield and Characterization of EVs
- 06:09Conclusion
This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.
