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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
 

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

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résumé
Automatic Translation

This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.

Tags

Purification High Yield Extracellular Vesicles Virus Concentration Separation Ultracentrifugation Nanoparticle Preparation Optimization Viral Infections HTLV Ebola Zika Culture Supernatant Late Log Cells Centrifugation Filtration PEG Precipitation Reagents
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