Here, we present a protocol to generate high-quality, large-scale transcriptome data of single cells from isolated human pancreatic islets using a droplet-based microfluidic single-cell RNA sequencing technology.
Xin, Y., Adler, C., Kim, J., Ding, Y., Ni, M., Wei, Y., Macdonald, L., Okamoto, H. Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets. J. Vis. Exp. (149), e59866, doi:10.3791/59866 (2019).