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Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
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遗传学
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JoVE 杂志 遗传学
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

DOI:
10.3791/59866-v

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11:34 min

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July 18, 2019

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, , , , , , ,
1Regeneron Pharmaceuticals, Inc

Chapters

  • 00:04Title
  • 01:01Human Islet Dissociation
  • 02:30Single Cell Suspension Quality Control and Single Cell Partitioning
  • 04:10Single Cell cDNA Amplification
  • 06:19Library Sequencing
  • 08:11Read Alignment and Data Analysis
  • 09:53Results: Dissociation, Single-cell Emulsion, cDNA Library, and Cell Types
  • 11:09Conclusion

Summary

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Here, we present a protocol to generate high-quality, large-scale transcriptome data of single cells from isolated human pancreatic islets using a droplet-based microfluidic single-cell RNA sequencing technology.

Tags

Single-cell RNA SequencingHuman Pancreatic IsletsCell HeterogeneityRare Cell TypeGene RegulationDissociationCell SuspensionEmulsionCell CountCell ViabilityMicrofluidic Chip

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