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Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
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Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

6,519 Views

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09:25 min

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May 21, 2019

DOI:

10.3791/59695-v

DOI:
10.3791/59695-v

•

09:25 min
May 21, 2019

•

26 Views
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1Department of Animal and Poultry Sciences,Virginia Polytechnic Institute and State University, 2School of Neuroscience,Virginia Polytechnic Institute and State University

Summary

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This protocol is designed to efficiently quantify ubiquitin-proteasome system (UPS) activity in different cellular compartments of the rodent brain. Users are able to examine UPS functioning in nuclear, cytoplasmic and synaptic fractions in the same animal, reducing the amount of time and number of animals needed to perform these complex analyses.

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Subcellular FractionationUbiquitin-proteasome SystemProtein UbiquitinationProteasome ActivityRodent BrainCytoplasmic FractionNuclear FractionSynaptic FractionCentrifugation

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Cite this Article

McFadden, T., Devulapalli, R. K., Jarome, T. J. Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain. J. Vis. Exp. (147), e59695, doi:10.3791/59695 (2019).

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