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Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
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Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

7,476 Views

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06:43 min

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June 20, 2019

DOI:

10.3791/59692-v

DOI:
10.3791/59692-v

•

06:43 min
June 20, 2019

•

3 Views
, , ,
1Laboratory for Neural Circuit Systems, Institute of Neuroscience,Tokushima Bunri University

Summary

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We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.

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Tominaga, Y., Taketoshi, M., Maeda, N., Tominaga, T. Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye. J. Vis. Exp. (148), e59692, doi:10.3791/59692 (2019).

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