Dergi
/
Nörobilim
/
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

7,461 Views

•

06:43 min

•

June 20, 2019

DOI:

10.3791/59692-v

DOI:
10.3791/59692-v

•

06:43 min
June 20, 2019

•

3 Views
, , ,
1Laboratory for Neural Circuit Systems, Institute of Neuroscience,Tokushima Bunri University

Özet

Automatically generated

We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.

Explore More Videos

Optical RecordingVoltage-sensitive DyeBrain SlicesWide-field Single-photonNeuropsychic DynamicsCircuit ChangesChemicalsBrain DevelopmentBrain ExtractionBrain SectioningVibratome SlicingBrain Slice RecoveryVoltage-sensitive Dye Staining

Read Article

Cite this Article

Tominaga, Y., Taketoshi, M., Maeda, N., Tominaga, T. Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye. J. Vis. Exp. (148), e59692, doi:10.3791/59692 (2019).

Download .ris file

Copy

Share Video

.

Copy