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小鼠视网膜冷冻节的制备,用于免疫性植物化学
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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
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小鼠视网膜冷冻节的制备,用于免疫性植物化学

DOI:
10.3791/59683-v

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05:25 min

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July 01, 2019

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, , ,
1Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Chapters

  • 00:04Title
  • 00:45Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
  • 03:08Sectioning Using a Cryostat
  • 03:51Results: Immunohistochemistry of Mouse Retinal Cryo-sections
  • 05:01Conclusion

Summary

자동 번역

자동 번역

本报告介绍了制备冷冻小鼠视网膜部分用于免疫组织化学(IHC)的综合方法。 所述方法包括眼部后杯的解剖、甲醛固定、嵌入最佳切割温度(OCT)介质和组织定向、切片和免疫染色。

Tags

Mouse RetinaRetinal DissectionImmunohistochemistryCryo-sectionEye EnucleationEye FixationCornea RemovalLens ExtractionRetina IsolationCryo-protectionSnap Freezing

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