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Masse massespektrometri-baserte Proteomikk analyser bruker OpenProt databasen å avsløre romanen proteiner oversatt fra ikke-kanoniske åpent lesing rammer
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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Masse massespektrometri-baserte Proteomikk analyser bruker OpenProt databasen å avsløre romanen proteiner oversatt fra ikke-kanoniske åpent lesing rammer

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07:38 min

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April 11, 2019

DOI:

10.3791/59589-v

DOI:
10.3791/59589-v

•

07:38 min
April 11, 2019

•

4 Views
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1Department of Biochemistry,Université de Sherbrooke, 2PROTEO,Quebec Network for Research on Protein Function, Structure, and Engineering

Summary

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OpenProt er en fritt tilgjengelig database som gjennomfører en polycistronic modell av eukaryote genomer. Her presenterer vi en protokoll for bruk av OpenProt databaser når avhør massespektrometri datasett. Med OpenProt gir database for analyse proteomic eksperimenter romanen og tidligere undetectable proteiner.

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Mass SpectrometryProteomicsOpenProt DatabaseNon-canonical Open Reading FramesNovel ProteinsProteomeProteomic AnalysesDatabase HandlingMS Convert ToolMzML File Format

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Brunet, M. A., Roucou, X. Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames. J. Vis. Exp. (146), e59589, doi:10.3791/59589 (2019).

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