/

/

伦蒂病毒介导基因在低附着板中制备的人类伪性沉默

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Please note that all translations are automatically generated. Click here for the English version.

伦蒂病毒介导基因在低附着板中制备的人类伪性沉默

DOI:

10.3791/59578-v

•

11:24 min

•

May 14, 2019

•

Siming Liu², Mikako Harata², Joseph A. Promes², Anthony J. Burand³, James A. Ankrum³, Yumi Imai²

¹Department of Internal Medicine, Carver College of Medicine,University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center,University of Iowa, ³Roy J. Carver Department of Biomedical Engineering,University of Iowa

Chapters

00:04Title
00:42Overnight Culture of Human Islets for Recovery After Shipment
01:32Preparation of Single Cell Suspension from Human Islets
04:03Pseudoislet Formation and Transduction by Lentivirus
08:44Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets
09:53Conclusion

Summary

자동 번역

提出了一种从分散的人类胰岛细胞中产生基因修饰的人类伪胰岛的函数,这种细胞由携带短发核糖核酸(shRNA)的慢病毒转导。该协议利用现成的酶和培养容器,易于执行,并产生适合功能和形态学研究的转基因人类假岛。

Tags

Lentiviral Gene Silencing Human Pseudoislet Low Attachment Plates Islet Function Gene Expression Modulation SHR8 Transduction Proteolytic Enzyme Collagenolytic Enzyme Cell Dissociation CMRL Medium Fetal Bovine Serum

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

在新鲜分离的人代绒毛滋养细胞转染和EMSA分析

在人原代细胞培养生物钟基因表达及荷尔蒙分泌的并行测量

饲养并在隐藏甲壳虫双链RNA介导的基因敲除，<em>白腹皮蠹</em

选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除

低丰度胚胎标本的染色质沉淀 (芯片) 协议

CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究

CRISPR/Cas9-mediated 目标集成在体内使用基于同源介导的端接连接策略

整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达

CRISPR 的染色质环结构重组

慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法

Read Article