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Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
JoVE Journal
Genetics
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JoVE Journal Genetics
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

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11:24 min

May 14, 2019

DOI:

11:24 min
May 14, 2019

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Summary

Automatically generated

A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.

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